Peritoneal lavage with ulinastatin reduced the expression levels of P-selectin protein in severe acute pancreatitis rats

Objective: To observe the effect and mechanisms of peritoneal lavage with ulinastatin on the inflammatory reaction and expression and staining intensity of P-selectin in SAP rats. Methods: Severe acute pancreatitis (SAP) model of rat was made by retrograde infusion of 5% sodium taurocholate into the pancreatic duct. After establishment of SAP model, intraperitoneal lavage was performed immediately in saline lavage group and peritoneal lavage with ulinastatin group, ulinastatin injection was also performed immediately in ulinastatin injection group. At 3 h after induction of SAP in all groups, TNF-α, and IL-10 and the expression levels, staining intensity of P-selectin protein of multiple organs in each group were detected. Results: Group IU and UL reduced the level of serum TNF-α and increased IL-10 effectively, Group UL exert the better effect. In Group SL, IU and UL, the expression levels and the staining intensity of P-selectin protein in the multiple organs were significantly lower than those in Group SAP and Group UL exert the best effect. Conclusions: Inflammatory condition could be well improved by intraperitoneal lavage with ulinastatin and may through inhibiting P-selectin protein expression in multiple organs.